[Effect of PPAR gamma His447His polymorphism on oocytes and fertilization in IVF]

Genetic factors play an important role in women fertility and embryonic development which may contribute to the efficacy of assisted reproduction techniques. The aim of this study was to investigate the effect of peroxisome proliferator-activated receptor gamma [PPAR gamma] His447His polymorphism on oocytes and fertilization in women undergoing IVF. Blood samples were obtained from 98 IVF patients referred to Tabriz Alzahra Hospital. Samples were analyzed for the PPAR gamma gene polymorphism using polymerase chain reaction-restriction fragment length polymorphism-based methods. Multivariate analyses were used to test the independence of associations between the number of mature oocytes and the number of oocytes fertilized as outcome variables and polymorphism of PPARy gene. Correlation analysis showed a significant inverse correlation between the age of women and the number of mature oocytes retrieved [r=-0.37, P<0.001] and oocytes fertilizaed [r=-0.25, P=0.015]. The ratio of the number of mature oocytes to oocytes fertilizaed was significantly [P<0.05] increased in carriers of the rare alleles than homozygous wild-type genotypes. The association of His447His polymorphism [P=0.003] remained statistically significant after adjustment for confounding factors in the multivariate analyses. This study presents evidences that the His447His polymorphism of PPAR gamma plays an important independent role in fertilization in vitro and thus possibly in female fertility

effect of aluminum on human erythrocyte glucose 6-phosphate dehydrogenase

Glucose 6-phosphate dehydrogenase [G6PD], the first enzyme in initiating the pentose phosphate shunt, is an important component in generation of NADPH. Although innumerable studies have been performed on human erythrocyte G6PD, however, the effect of trace elements on the enzyme activity requires further investigations. To study the effect of aluminum on human erythrocyte G6PD. In this experimental research, following the purification of G6PD using chromatographic methods, the effect of different concentrations of Al[3+] [up to 100 micro-molar] on G6PD activity was studied. The enzyme activity was measured at different concentrations of glucose 6-phosphate and NADP[+] to determine the type of inhibitory action. Aluminum at the concentration of 100 microM showed a considerable inhibitory effect on G6PD activity [60%]. The type of inhibitory action, depending on the use of glucose-6-phosphate or NADP[+], was competitive and noncompetitive, respectively. Aluminum exerts an inhibitory action on human erythrocyte G6PD activity